Selected Publications

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• R.K. MacNeal and D.L. Purich (1978) Stoichiometry and Role of GTP Hydrolysis in Bovine Microtubule Assembly, Journal of Biological Chemistry 253, 4683-4687. [Citation Format]
• T.L.Karr, H.D. White and D.L. Purich (1979) Characterization of Brain Microtubule Proteins Prepared by Selective Removal of Mitochondrial and Synaptosomal Components, Journal of Biological Chemistry 254, 6107-6111.
• T.L. Karr and D.L. Purich (1979) A Microtubule Assembly/Disassembly Model Based on Drug Effects and Depolymerization Kinetics after Rapid Dilution, Journal of Biological Chemistry 254, 10885-10888.
• B.J. Terry and D.L. Purich (1979) Nucleotide Release from Tubulin and Nucleoside 5'-Diphosphate Kinase Action in Microtubule Assembly, Journal of Biological Chemistry 254, 9469-9476. [Citation Format]
• T.L. Karr, A.E. Podrasky and D.L. Purich (1979) Participation of Guanine Nucleotides in Nucleation and Elongation Steps of Microtubule Assembly, Proceedings of the National Academy of Sciences USA 76, 5475-5479.[Citation Format]
• H.D. White, B.A. Coughlin and D.L. Purich (1980) Adenosine Triphosphatase Activity of Bovine Brain Microtubule Protein, Journal of Biological Chemistry 255, 486-491.
• D.L. Purich and R.D. Allison (1980) Isotope Exchange Methods for Elucidating Enzymic Catalysis, in "Enzyme Kinetics and Mechanism", Part B,Methods in Enzymology, vol. 64, Academic Press, New York, 1-46. [Citation Format]
• T.L. Karr and D.L. Purich (1980) A Rapid Dilution Cuvette for Kinetic Studies of Microtubule Disassembly, Analytical Biochemistry 104, 311-314.
• B.J. Terry and D.L. Purich (1980) Assembly and Disassembly Properties of Microtubules Formed in the Presence of GTP, 5'-Guanylyl Imidodiphosphate, and 5'-Guanylyl Methylene diphosphate, Journal of Biological Chemistry 255, 10532-10536. [Citation Format]
• T.L. Karr, D. Kristofferson and D.L. Purich (1980) Mechanism of Microtubule Depolymerization: Correlation of Rapid Induced Disassembly Experiments with a Kinetic Model for Endwise Depolymerization, Journal of Biological Chemistry 255:18, 8560-8566 (includes a miniprint supplement on the kinetics of cold-induced depolymerization using a rapid heat-exchanger) [Citation Format].
• D. Kristofferson, T.L. Karr and D.L. Purich (1980) Dynamics of Linear Protein Polymer Disassembly, Journal of Biological Chemistry 255, 8567-8572 [Citation Format].
• T.L. Karr, D. Kristofferson and D.L. Purich (1980) Calcium Ion Induces Endwise Depolymerization of Bovine Brain Microtubules, Journal of Biological Chemistry 255, 11853-11856 [Citation Format].
• D. Kristofferson and D.L. Purich (1981) A Quantitative Treatment of Reversible Monomer-Polymer Exchange Reactions with Microtubules and Other Biopolymers, Journal of Theoretical Biology 92, 85-96.
• D.L. Purich, B.J. Terry, H.D. White, B.A. Coughlin, T.L. Karr and D. Kristofferson (1981) Microtubule Associated Protein Phosphorylation and Calcium Ion Regulation of Bovine Brain Microtubule Self-Assembly, vol. 8, Protein Phosphorylation, (0. M. Rosen and E.G. Krebs, eds.) Cold Spring Harbor Conferences on Cell Proliferation, 1143-1155.
• D. Kristofferson and D.L. Purich (1981) Time Scale of Microtubule Length Redistribution, Archives of Biochemistry and Biophysics 211, 222-226.
• W.C. Thompson, L. Wilson and D.L. Purich (1981) Taxol Induces Microtubule Assembly at Low Temperature, Cell Motility l, 445-454.
• A. Sanders, D.L. Purich and D.S. Cannell (1981) Oxygenation of Hemoglobin: Correspondence of Crystal and Solution Properties Using Translational Diffusion Constant Measurements, Journal of Molecular Biology 147, 583-595.
• D. Kristofferson, S.-H. Lee, B.J. Terry, A. Cimino Saucier, T.L. Karr and D.L. Purich (1982) Evaluation of Potential Roles for GDP in Microtubule Assembly and Disassembly in Biological Functions of Microtubules and Related Structures (H. Mohri, H. Sakai, and G. G. Borisy, eds.), Academic Press, New York, 49-60.
• B.J. Terry and D.L. Purich (1982) Nucleotide-Dependent Enzymes in Microtubule Systems, Advances in Enzymology (A. Meister, ed.) 53, 113-161.
• D.L. Purich and D. Kristofferson (1984) Microtubule Assembly: A Review of Progress, Principles and Perspectives, Advances in Protein Chemistry (C. B. Anfinsen, J. T. Edsall and F. M. Richards, eds.) vol. 36, Academic Press, New York, 133-212.
• D.G. Neal, D.L. Purich and D.S. Cannell (1984) Osmotic Susceptibility and Diffusion Coefficient of Charged Bovine Serum Albumin, Journal of Chemical Physics 80, 3469-3477.
• D.L. Purich and R.M. Scaife (1985) Microtubule Cytoskeletal Proteins as Targets for Covalent Interconverting Enzymes, Current Topics in Cellular Regulation 27, 107-116.
• L. Wilson, H.P. Miller, K.W. Farrell, K.B. Snyder, W.C. Thompson and D.L. Purich (1985) Taxol Stabilization of Microtubules In Vitro: Dynamics of Tubulin Addition and Loss at Opposite Microtubule Ends, Biochemistry 24, 5254-5262. [Citation Format]
• D.J. Asai, W.C. Thompson, L. Wilson, C.F. Dresden, H. Schulman and D.L. Purich (1985) Microtubule-Associated Proteins (MAPs): A Monoclonal Antibody to MAP-1 Decorates Microtubules In Vitro But Stains Stress Fibers and Not Microtubules In Vivo, Proceedings of the National Academy of Sciences USA 82, 1434-1438. [Citation Format]
• P.S. Yamauchi and D.L. Purich (1987) Modulation of Microtubule Assembly and Stability by Phosphatidyl Inositol Action on Microtubule-Associated Protein-2, Journal of Biological Chemistry 262, 3369-3375. [Citation Format]
• G. Flynn and D.L. Purich (1987) GTP Regeneration Influences Interactions of Microtubules, Neurofilaments, and Microtubule-Associated Proteins In Vitro, Journal of Biological Chemistry 262, 15443-15447. [Citation Format]
• G. Flynn, J.C. Joly and D.L. Purich (1987) The 28,000 Mr Microtubule-Binding Domain of Microtubule-Associated Protein-2 Also Contains A Neurofilament-Binding Site, Biochemical and Biophysical Research Communications 148, 1453-1459. [Citation Format]
• D.L. Purich and R.M. Scaife (1987) Enzymatic Modulation of Cytoskeletal Self-Assembly: ADP Ribosylation of Microtubule Protein Components, Enzyme Dynamics and Regulation (P.B. Chock, C.Y. Huang, C.L. Tsou, and J.H. Wang, eds.) Springer-Verlag, New York, 217-223.
• J.C. Joly, G. Flynn and D.L. Purich (1989) The Microtubule-Binding Fragment of Microtubule Associated Protein-2: Location of the Protease-Accessible Site and Identification of an Assembly-Promoting Peptide, Journal of Cell Biology 109, 2289-2294. [Citation Format]
• J.M. Angelastro and D.L. Purich (1990) Apparently Irreversible GTP Hydrolysis Attends Tubulin Self-Assembly, European Journal of Biochemistry 191, 507-511. [Citation Format]
• J.C. Joly and D.L. Purich (1990) Peptides Corresponding to the Second Repeated Sequence in MAP-2 Inhibit Binding of Microtubule-Associated Proteins to Microtubules, Biochemistry 29, 8916-8920.[Citation Format]
• R.M. Scaife, L. Wilson and D.L. Purich (1992) Microtubule Protein ADP-Ribosylation In Vitro Leads to Assembly Inhibition and Rapid Depolymerization, Biochemistry 31, 310-316. [Citation Format]
• N. Raffaelli, P.S. Yamauchi and D.L. Purich (1992) Microtubule-Associated Protein Autophosphorylation Alters In Vitro Microtubule Dynamic Instability, FEBS Letters 296, 21-24. [Citation Format]
• N. Raffaelli, R.M. Scaife and D.L. Purich (1992) ADPRibosylation of Chicken Red Cell Tubulin and Inhibition of Microtubule Self-Assembly In Vitro by the NAD+-Dependent Avian ADP Ribosyl Transferase, Biochemical and Biophysical Research Communications 184, 414-418. [Citation Format]
• N.L. Deans, R.D. Allison and D.L. Purich (1992) Steady-State Kinetic Mechanism of Bovine Brain Tubulin: Tyrosine Ligase, Biochemical Journal 286, 243-251. [Citation Format]
• A.M. Ainsztein and D.L. Purich (1992) Bovine Brain Microtubule-Associated Protein-2 Cleavage by Human Immunodeficiency Virus Proteinase, Journal of Neurochemistry 59, 874-880. [Citation Format]
• J.M. Angelastro and D.L. Purich (1992) Adenine and Guanine Nucleotide Content of Triton Extracted Cytoskeletal Fractions of Nonmuscle Cells, Analytical Biochemistry 204, 47-52.
• P.A. Antinozzi, C.M. Brown and D.L. Purich (1992) Calcium Oxalate Monohydrate Crystallization: Citrate Inhibition of Nucleation and Growth Steps, Journal of Crystal Growth 125, 215-222.
• J.M. Angelastro and D.L. Purich (1992) 2'-dGTP in the Microtubule Cytoskeleton of Neuronal Cells Cultured with Nerve Growth Factor, Journal of Biological Chemistry 267, 25685-25689. [Citation Format]
• P.S. Yamauchi, G. Flynn, R.L. Marsh and D.L. Purich (1993) Reduction in Microtubule Dynamics In Vitro by Brain Microtubule-Associated Proteins and by a Microtubule-Associated Protein-2 Second Repeat Sequence Analogue, Journal of Neurochemistry 60, 817-826. [Citation Format]
• P.S. Yamauchi and D.L. Purich (1993) Microtubule-Associated Protein Interactions with Actin Filaments: Evidence for Differential Behavior of Neuronal MAP-2 and Tau in the Presence of Phosphatidyl-Inositol, Biochemical and Biophysical Research Communications 190, 710-715. [Citation Format]
• P.S. Yamauchi and D.L. Purich (1993) Absence of 2'-Deoxy-GTP in Adult Brain Tubulin, Biochemical and Biophysical Research Communications 192, 268-272 [Citation Format].
• D.L. Purich and J.M. Angelastro (1994) Microtubule Dynamics: Bioenergetics and Control, Advances in Enzymology (A. Meister, ed.) 69, 121-154.
• J.M. Angelastro and D.L. Purich (1994) Phosphorylation States of Actin Filament Adenine Nucleotides in Detergent-Extracted Neuronal Cytoskeletal Fraction, Biochemical & Biophysical Research Communications, 201, 1490-1494.[Citation Format]
• F.S. Southwick and D.L. Purich (1994) Arrest of Listeria Movement in Host Cells by a Bacterial ActA Analogue: Implications for Actin-Based Motility, Proceedings National Academy of Sciences U.S.A. 91, 5168-5172. [Citation Format]
• C.M. Brown, F. Novin, and D.L. Purich (1994) Calcium Oxalate Crystal Morphology: Influence of Phospholipid Micelles with Composition Based on Each Leaflet of the Erythrocyte Membrane, Journal of Crystal Growth, 135, 523-532.
• F.S. Southwick and D.L. Purich (1994) Dynamic Remodeling of the Actin Cytoskeleton: Lessons Learned from Listeria Locomotion, BioEssays, 16, 885-891.[Citation Format]
• R.L. Coffey, J.C. Joly, B.D. Cain and D.L. Purich (1994) Exploring the Microtubule-Binding Region of Bovine Microtubule-Associated Protein-2: cDNA Sequencing, Bacterial Expression and Site-Directed Mutagenesis, Biochemistry, 33, 13199-13207.[Citation Format]
• A.M. Ainsztein and D.L. Purich (1994) Stimulation of Tubulin Polymerization by MAP-2: Control by Protein Kinase C-Mediated Phosphorylation at Specific Sites in the Microtubule-Binding Region, Journal of Biological Chemistry, 269, 28465-28471 [Citation Format]
• R.L. Coffey and D.L. Purich (1995) Non-Cooperative Binding of the MAP-2 Microtubule Binding Region to Microtubules, Journal of Biological Chemistry, 270, 1035-1040.[Citation Format]
• F.S. Southwick and D.L. Purich (1995) Inhibition of Listeria Locomotion by Mosquito Oostatic Factor, a Natural Oligoproline Peptide Uncoupler of Profilin Action, Infection & Immunity, 63, 182-190[Citation Format].
• W. Zeile, D. L. Purich, and F. S. Southwick (1996) Oligoproline Sequence Recognition in Profilin-Mediated Acceleration of Actin-Based Shigella Motility, Journal of Cell Biology, 133, 49-59. [Citation Format]
• F.S. Southwick and D.L. Purich (1996) Intracellular Pathogenesis of Listeriosis, New England Journal of Medicine 334, 770-776.
• E.Y. Zhang, M.A. DeTure, M.R. Bubb, T.L. Caviston, G.W. Erdos, S.D. Whittaker, and D.L. Purich (1996) Self-Assembly of the Brain MAP-2 Microtubule-Binding Region into Polymeric Structures Resembling Alzheimer Filaments, Biochemical & Biophysical Research Communications, 229, 176-181.
• M. DeTure, E. Zhang, M.R. Bubb, and D.L. Purich (1996) Assembly of MAP-2c and MTBR Fragments into Polymers Resembling Paired Helical Filaments, Journal of Biological Chemistry, 271, 32702-32706.
• D.L. Purich and F.S. Southwick (1997) ABM-1 and ABM-2 Homology Sequences: Consensus Docking Sites for Actin-Based Motility Defined by Oligoproline Regions in Listeria ActA Surface Protein and Human VASP, Biochemical & Biophysical Research Communications, 231, 686-691.
• F. Kang, R.O. Laine, M.R. Bubb, F.S. Southwick, and D.L. Purich (1997) Profilin Interacts with the GPPPPP Sequences of Vasodilator- Stimulated Phosphoprotein (VASP): Implications for Actin-Based Listeria Motility, Biochemistry, 36, 8384-8392.
• D.L. Purich (1998) Advances in the Enzymology of Glutamine Synthesis.  Advances in Enzymology (D.L. Purich, ed.) vol. 72, 9-42.
• L. DiNoto, M. DeTure, and D.L. Purich (1999) Disulfide-linked MAP2-MAP2 Dimers Still Promote Tubulin Polymerization and Stabilize Microtubules, Molecular Cell Biology Research Communications, 2, 71-76.
• D.L. Purich & F.S. Southwick (1999) Energetics of Nucleotide Hydrolysis in Polymer Assembly/Disassembly: The Cases of Actin and Tubulin "Enzyme Kinetics & Mechanism, Part E" Methods in Enzymology, (V.L. Schramm and D.L. Purich, eds.), 308, 93-110.
• F.S. Southwick and D.L. Purich (1999) Actin-Based Motility of the Intracellular Pathogen Listeria monocytogenes: Assessing the Inhibitory Specificity of ABM-1 S Peptide Analogues, Molecular Cell Biology Research Communications, 1, 176-181.
• W.L. Zeile, D.L. Purich, and F.S. Southwick (2000) Video Microscopy: Protocols for Examining the Actin-Based Motility of Listeria, Shigella, Vaccinia, and Lanthanum-Induced Endosomes, Laboratory Protocols in Cell Biology, 7, 125-158.
• F. Kang, D.L. Purich, and F.S. Southwick (1999) Profilin Promotes Barbed-End Actin Filament Assembly without Lowering the Critical Concentration, J. Biol. Chem., in press.