Physical Biochemistry/ Structural Biology-- Concepts
Protein primary, secondary, ternary and quaternary structure.
Protein folding and unfolding
Prediction of protein secondary structure.
Ramachandran diagrams
Nucleic acid structure- B, A, Z, C, psi, bent, kinked and supercoiled DNA, RNA II, tRNA
Helix-coil transition of nucleic acids, denaturation, hybridization
RNA secondary structure predictions
Nucleic acid chemistry- chemistry of modification, covalent interactions, heavy metals (Hg, Ag, Pb, Cu), intercalation, binding of water and ions
Nucleic acid-protein interactions--general considerations of specificity, general binding, helix-turn-helix, Zn finger, leucine zipper, helix-loop-helix, pou
Electrostatics of protein and nucleic acid interactions
Enzyme kinetics
Thermodynamics of biological systems
Enzymology
Membrane biophysics
Fluidity in biological systems
Physical Biochemistry/ Structural Biology-- Methods
UV/Visible spectroscopy
IR and Raman spectroscopy
Fluorescence, including quenching and energy transfer
Circular dichroism
Light scattering
Sedimentation- velocity, equilibrium, density
Viscosity
Electrophoresis- SDS PAGE, native, 2-D, agarose, isoelectric focusing, pulse field, gel shift, capillary
Osmometry and dialysis
Biochemistry
Carbohydrate Structure and Chemistry
Glycolysis
Gluconeogenesis
Glycogen -- Synthesis and Degradation
TCA Cycle and Pentose Phosphate Shunt
Respiratory Chain
Oxidative Phosphorylation
Cell Biology
Visual transduction pathway
G-proteins
Cyclic nucleotide regulation: bacteria, fungi and higher systems
Plant and fungal cell wall - structure, biochemistry and regulation
Photomorphogenesis
Phycomyces blakesleeanus
Development in filamentous fungi, Dictyostelium and C. elegans
Hormonal and neurotransmitter signal transduction
Channels and pores in biomembranes
Signal transduction in microorganisms-especially light
Calmodulin and calcium signalling
Bacterial chemotaxis
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