Mammalian Mitochondrial Ribosomal Consortium
 
| Consortium Home | Purpose | Participants | Research Presentations | Publications | Links | Members Only | 

Progress in sequencing of individual bovine MRPs

Individual proteins, numbered according to the standard nomenclature system for bovine mitoribosomal proteins (Matthews, et al., J. Biol. Chem. 257:8788-8794, 1982) are colored blue if they have been sequenced.
 

Small subribosomal particle  (MRP-Snn)                      Large subribosomal particle (MRP-Lnn)

 
 


Human MRPs identified

Human MRP
E. coli family
N-terminal sequence
experimentally determined
N-terminal sequence species
Human genomic clone available
Human MRP gene mapped†
Human chromosome locus
Candidate Disease

Mouse/ Human
               
MRP-L2
none
Yes
Bovine
Yes
      
MRL3
L3
No
  
Yes
      
MRP-L3
none
Yes
Bovine
Yes
Yes-RH
17q23
js/ RSS
MRP-L5
none
Yes
Bovine
Partial
      
MRP-L7
L15
Yes
Bovine
No
      
MRP-L8
L10
Yes
Rat
Yes
      
L11-hom
L11
No
          
MRPL12
L7/L12
Yes
Bovine
Yes
Yes-FISH
17q25
/RSS, DFNA20
MRP-L14
L2
Yes (internal)
Bovine
No
      
MRP-L15
L19
Yes
Bovine
Yes
      
MRP-L18
L24
Yes 
Bovine
        
MRP-L22
none
Yes
Rat
Yes
      
MRP-L25
L22
Yes
Rat
Yes
      
MRP-L26
L17
Yes
Bovine
No
      
MRP-L27
  
Yes
Rat
No
      
MRP-L28
L30
Yes
Rat
Yes
      
MRP-L31
none
Yes
Rat
Yes
      
MRP-L32
L14
Yes
Rat
Yes
      
MRP-S4
none
Yes
Bovine
No
      
MRP-S5
none
Yes (internal)
Bovine
Yes
      
MRP-S6
S9
Yes
Bovine
No
      
MRP-S8
none
Yes
Bovine
No
      
S11-hom
S11
No
          
RPMS12
S12
No
  
Yes
Yes-RH
19q13.1
qv,nv/ DFNA4
MRP-S12
none
Yes
Bovine
No
      
MRP-S13
none
Yes
Bovine
Yes
      
HSMRPS14
S14
No
  
Yes
      
MRP-S23
S10
Yes
Bovine
        
MRP-S25
none
Yes
Bovine
Yes
Yes-RH
7p14 
Hoxa1/ RSS
MRP-S28
none
Yes
Bovine
No
      
MRP-S29
S6
Yes
Bovine
No
      
               
†-FISH, fluorescence in situ hybridization; RH, Radiation Hybrid mapping of EST or cDNA. 

RSS:  Russell-Silver Syndrome, also called Russell-Silver Dwarfism, a dwarfism characterized by low birth weight and lateral asymmetry.
 
 
 

Table 1. Molecular weights and isoelectric points of small subunit proteins from human mitochondrial ribosomes (HGNC nomenclature), compared to E. coli ribosomal proteins

E.coli Small subunit
Human MRPSs
Protein
mass(kDa)
pI
Protein 
mass(kDa)
pI
Yeast MRP?
S1
61
4.8
S2
26.7
7.1
MRPS2 a
30.1
9.1
MRP4p
S3
26
10.9
Var1p
S4
23.4
10.6
NAM9p
S5
17.6
10.8
MRPS5c
38.8
10.6
MRPS5
S6
15.1
5.3
MRPS6 a
13.8
9.5
MRP17
S7
20
10.9
MRPS7 f
24.3
9.6
Rsm7p
S8
14
10.1
YMR158w
S9
14.8
11.3
MRPS9c
40.4
9.9
MRPS9
S10
11.7
10.3
MRPS10 g
17.7
6.2
S59279
S11
13.8
11.5
MRPS11 a
20.7
11.2
MRPS18
S12
13.7
11.3
MRPS12 h
15.1
10.9
P53732
S13
13.1
11.2
Pet123p
S14
11.5
11.4
MRPS14 g
15.1
11.5
MRP2
S15
10.2
10.9
MRPS15a
27.9
10.9
MRPS28
S16
9.2
11
MRPS16a
14.3
10.2
YPL013C
S17
9.7
10.3
MRPS17a
14.5
10.5
YMR188C
S18
8.9
11
MRPS18Aa,b
22
10.9
MRPS18B a,b
29
9.9
MRPS18Ca,b
15
10.2
YER050C
S19
10.4
11.2
YNR037c
S20
9.6
11.6
S21
8.5
11.4
MRPS21a
9.3
10.3
YBL090W
MRPS22 g
35.2
6.4
MRPS23 g
17.6
9.5
YIL093C
MRPS24 g
15.3
9.9
MRPS25 g
20
9.4
YKL167C
MRPS26 g
21.3
10.2
MRPS27 d
25.7
10.5
MRPS28 e
13.1
8.2
MRPS29 i
42.6
9
YGL129C
MRPS30 i
47.7
7.3
MRPS31a
39
10
MRPS32a
16.7
8.9
MRPS33a
12.6
10.8
Rsm27p
MRPS34 j
44.6
5.4
MRPS35 k
34.5
8
YDR175C
MRPS36a
10.2
10.3
YMR-31

a Koc et al., 2001b

b MRPS18A, B, C are MRPS18 3, 2, 1 in Koc et al., 2001b 

c Koc, et al., 2001a

d MRPS34 in Koc et al., 2001b

e MRPS35 in Koc et al., 2001b

f Koc, et al., 1999

g Koc, et al., 2000

h Suzuki, et al., 2001a

i Koc, et al., 2001d

j MRPS27 in Koc, et al., 2001b

k MRPS28 in Koc, et al., 2001b